BTLA antibody (FineTest®647)

Catalog No. ABIN7675483

The Mouse Monoclonal anti-BTLA antibody has been validated for FACS. It is suitable to detect BTLA in samples from Mouse.

Quick Overview for BTLA antibody (FineTest®647) (ABIN7675483)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BTLA antibody is conjugated to FineTest®647

Application: Flow Cytometry (FACS)

Clone: 6F7

Product Details anti-BTLA Antibody (FineTest®647)

Purpose: FineTest®647 Anti-Mouse CD272 Antibody

Isotype: IgG1, kappa

Application Details

Application Notes: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0. 1 % sodium azide, 1 %BSA, pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: 4 °C

Storage Comment: 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: CD272

Background:

Synonyms: BTLA|B and T lymphocyte attenuator

Background: B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, reduced effectiveness of T regulatory cells. BTLA takes an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naive T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4. But further studies are needed to confirm. The existence of three distinct BTLA alleles was reported. The BTLA antibody reacts with mouse BTLA from both BALb/c and C57BL/6 strains.

Molecular Weight: 0

Gene ID: 208154

UniProt: Q7TSA3

Pathways: Cancer Immune Checkpoints